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牛心朴子、四合木和鄂尔多斯半日花的杀线机制(英文)



全 文 :第 34卷 第 4期
2010年 7月
南京林业大学学报(自然科学版)
JournalofNanjingForestryUniversity(NaturalScienceEdition)
Vol.34, No.4
Jul., 2010
htp://www.nldxb.com
IUFRO2009国际松萎蔫病研讨会学术论文摘要选登
牛心朴子 、四合木和鄂尔多斯半日花的杀线机制
杨 静 ,刘 强
(天津师范大学生物系 ,天津 300387)
摘要:牛心朴子对线虫的杀线活性的研究证实 ,牛心朴子甲醇提取物对松材线虫的杀线活性随时间
和提取物浓度增加明显增加;紫外线和加热处理会使其提取物失活 ,提取物的新鲜度会影响其活
性。分析认为牛心朴子的毒性机理是干扰线虫葡萄糖和蛋白质代谢 ,抑制 SOD活性 ,诱导 POD和
CAT活性 ,进而产生自由基摧毁保护酶系统 ,同时抑制 AChE活性 ,破坏神经系统 ,抑制 GST活性 ,
并干扰解毒酶系统的代谢。四合木的毒性机理是干扰线虫葡萄糖和蛋白质代谢 ,抑制 SOD活性 ,
诱导 POD和 CAT活性 ,进而产生自由基或改变酶分子 ,这样就会导致损毁保护酶系统 ,对 AChE和
AKP的作用类似牛心朴子的情况。鄂尔多斯半日花的毒性机理是干扰线虫葡萄糖和蛋白质代谢 ,
抑制 SOD活性 ,诱导 POD和 CAT活性 ,进而产生自由基摧毁保护酶系统 ,同时抑制 AChE活性 ,破
坏神经系统。
关键词:杀线机制;牛心朴子;四合木;鄂尔多斯半日花
中图分类号:S763    文献标志码:D    文章编号:1000-2006(2010)04-0172-02
ThenematocidalmechanismofCynanchumkomarovi, Tetraenamongolica
andHelianthemumordosicum
YANGJing, LIUQiang
(DepartmentofBiology, TianjinNormalUniversity, Tianjin300387, China)
  Thenematicidalactivity[ 1] ofCynanchumkomarovii[ 2] and
thepoisoningmechanism[ 3-5] ofC.komarovii, Tetraenamon-
golica[ 6-8] andHelianthemumordosicum[ 9-13] againstBursaphe-
lenchusxylophiluswerestudied.Theresultsshowedthata
methanolextractofC.komaroviihadthegreatnematicidalactiv-
ity, andtheactivityoftheextractincreasednoticeablyover
timeanddosage.Theactivityoftheextractwasunstableunder
theUV[ 14-16] andheattreatment[ 17] , andthematerialsfresh-
ness[ 18] couldafectitsactivity.
ThepoisoningmechanismoftheC.komaroviiwasthought
tobetheresultofinterferencewiththemetabolismofglucose
andprotein;inhibitedSODactivity, inducedPODandCATac-
tivity, leadedthefreeradicaltodestroytheprotectiveenzyme
system;inhibitedAChEactivity, destroyedthenervoussystem;
inhibitedGSTactivity, andinterferencewiththemetabolismof
detoxifyingenzymes.
ThepoisoningmechanismoftheT.mongolicawasthere-
sultofinterferencewiththemetabolismofglucoseandprotein;
inhibitedSODandCATactivity, inducedthefreeradicalorthe
changeofenzymemolecules, whichleadedtothedamageof
protectiveenzymesystem;theeffectontheAChEandAKPis
thesametotheC.komarovi′s.
ThepoisoningmechanismoftheH.ordosicuminterfered
withthemetabolismofglucoseandprotein;inhibitedSODac-
tivity, inducedPODandCATactivity, leadedthefreeradical
todestroytheprotectiveenzymesystem;inhibitedAChEactivi-
ty, destroyedthenervoussystem.
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IUFRO2009国际松萎蔫病研讨会学术论文摘要选登
伞滑刃属(Bursaphelenchus)属内分类研究
HelenBraasch1 ,顾建锋 2 , WolfgangBurgermeister3
(1.德国农林生物研究中心检疫办公室 , Kantstrasse5, D-14471Potsdam, Germany;2.宁波出入境检验检疫局 ,
浙江 宁波 315012;3.德国 JuliusKuehn流行病接病原诊断研究所 , Messeweg11, D-38104 Braunschweig, German)
摘要:目前已经报道了伞滑刃属(Bursaphelenchus)内近百种线虫 ,其共同的形态特征是雄虫的尾
部末端具有交合伞。在该属的分类中尚存在同物异名的问题 。为此 ,基于其特征的复杂性 ,笔者
提出根据线虫侧线的数目 、尾乳突的排列 、是否出现阴门盖 、交合刺形状 、外分泌孔及后部子宫分
支的位置等特征 ,将该属分为 7组 ,其中 4条侧线组为 xylophilus, okinawaensis, africanus, fungi-
vorus, cocophilus, kevini和 sexdentati;3条侧线组为 eggersi, eremus, hofmanni, leoni;2条侧线组为
abietinus和 sinensis。 73种列入上述分组中 , 24种因为缺乏形态学资料未能列入。交合刺不能用
于分组 。列入该分组的 39种基于亚基核糖体 DNA数据的分子树说明了该分组的合理性 。另
外 , Parasitaphelenchinae, Aphelenchoidinae和 Ektaphelenchinae亚科中 28种线虫基于核糖体大亚
基 D1—D2区序列生成的分子树显示了它们在 Aphelenchoididae科中的系统关系。
关键词:伞滑刃属;属内分组;侧线数目;分子树
中图分类号:S763    文献标志码:D    文章编号:1000-2006(2010)04-0173-02
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