全 文 ：牛态科学2003年05月第22卷第2期 !!旦!竺!!!!!!!型!!竺!：!!竺!：!!【!!!11三二堕
连香树科及其近缘植物m口水序列分析和系统学意义
章 群1，聂湘平1，施苏华扩，黄椰林2，谈凤笑2，张宏达2(】暨南大学科学技术学；皖，广
州5】()632：2中山大学科学学院，广州510275
【摘要】 测定和分析了连香树科(cercidiPhyllaceaej、交让木科(Daphniphyllaceae)、金缕梅科(HamameIidaceac)代表
植物的Hf绿体，阳啦序列(5’端31bps除外)，以木兰属作为外类群，应用邻接法构建分子系统树，结果表明：连香杠f
科与水青树科的亲缘关系较远。连香树科、交让木科和金缕梅科形成了一个自展数据主持率(boots咖p)为100％的单
系类群，其中金缕梅科枫香属(L由“讹小6口r)、红花荷属(舶。出k旭)和金缕梅属(mmd口圯m)虽构成了·个学系茧
群．但自展数据支持率仅为68％：连香树科与交让木科构成的单系分支自展数据支持率仅为53％。由于连香料科、受
让术科、金缕梅科之间的进化距离相当短，表明这3个科之间亲绿关系密切，内部分支的自展数据支持率不商表州
它们之间准确的亲缘关系有待进一步研究。本研究结果与而cL、aptB、I8srDNA序列分析结果相似。但自展数据支社
率更高，表明，糊fK序列分析可应用于较高等级分类群系统发育关系的研究。
关键词：连香树科；交让术科：金缕梅科：，船fK；系统发育
中圈分类号：0949．746 文献标识码：A 文章编号：1008—8873【2003)02一113一【)4
Phylogene恤AmIljti钨0fCerdd王phyU舵e鼬B躯edon删fKSequenc船，ZANGQun，NIEXi粕g—pin玉SHISu-hua
HUANGYc—lin，1州Feng—xia0，GHANGHong—da(Collegeof ifescience柚dtcchn010zⅥJinanUniversitv，Guang，h(’l}
510632，Schooloflifescienccs．zhon譬shaIlUniv∽；tMGu柚gzhou510275，china)
AbstraetTbfuftherresolvethephylogenedcamnidesofCercidjphvllaceae，m U(genesequencesgcncratcdfor
TeⅡ∞centraceae，Daphni口hyllaceae，Cercidiphyllaccae粕dL矾池删如E鼢od口括如and，妇小dmP，括ofHalnamelidaceac，werc
analvzodu时ngNeizhbOr_Joiningme血Odw th朋矗窖，坩妇部粕outgrou口．The脚rKconsensust eedisplayssirmlartopoIogy“，
thoseof帕d。。atDB，and18S—)NAsequenoesanalysesrespectivelvandcombined、撕thlg℃aterphylogenedcresoluuonl￡
showst11atCerddiDhvllaceae，Da口hni口hyUiaceae锄dHam锄elidaceaefo玎nBdarobustclade，butrda60nshjpsamong山elnan-
notresolved；thephylogBn枷cTcladonshipsofTb姐cen廿ace∞t0Cercidiphyllaceaearcnotsoclosedas订adl“onal∽atmen‘
Thus，severallinesof州dence．de^vedfmmbOtll血echIOroplast且enorrlea11d18SDNAsequences，suggestLhepresenceof
considerably口bylogenedcamnm sb tween(轴℃idiphyllac∞e，Da口lllli口hyIlaceae，趴dHarll melidaceae，funherinfonnam¨
onsomeom盯quicl(】y~ev酣vedg nesarDeededtD删Vephy】ogenedcreJa廿舢sbjps圳ongt11跚Thestudyc唧bo阳lesIhI‘
ideathat，ndfKscqucnceshavcpoten廿a1．nresolvingphylogenya“aIIlilylevel_
Ke，words：o∞cidiphyllaceae：DaphIliphyllaceae；HaIllaInelldaceae；matK；Phylogeny
TheCercidiphyllaceaeis n stemAsiatic
monogenencf删ly，contaimngtwOspecies，inp teof
numerousmorphological，anatoIIlical，p yn0109ical，
cvtological；uldphytocheIllicalstudies，血eaffininesof
thefamilvwerepoorlyunders幻odan exⅡemely
pfoblematic．It hasbeenplacedin (1)血e
Tr()chodendmlesbyDa|llgren⋯aIldEndress“。；(2)inthe
Ham啪elidalesbyCronquist”1：(3)intheSax 疔agales
byAPG”1；(4)asaseparateordeLCercidiphyllales，in龇
Hamamelidaeby Takh喇an”1．Halnerplaced
“CercidiphvllumasasubfamilyCercidiphylleaebefm
血e Bucklandioideae”扣1．Acladis龀a11aIvsisoft|Ie
“lower’Hama|nendaesuggest dm tCercidiphyllumare
relative(1fH锄amelidaceae”1．oner asonf ra1ackof
agreementaboutrelationshipsmaybethata la曙e
numberofnoralcharactershaveevolvedmparaUel．an
hypothesisbasedupondataotherthanmorphologicalor
chromosomaJchaI cterswould．therefore。beofvaluable
foranalvsesofamnitiesofmefa血lv．Thebroad
analysesof岫也，and18SrDNA，印msequences
sug叠estedthatCercidiphyUace∞arerela叫to
Ham蛐elidaceae，DaphniphyUaceaeetc”⋯1Althou譬h
帕cLsequencedalacaIlpm“desomeinfonnation
concemin譬Cercidiphyllaceae，thatinfomla石onw sno
sufficienttores01verelationshipsarnon譬tbefdnljlies，
increasedsampHng吖血esef撕Ⅱesbasedon小(I。still
f缸1edto“eld毋。eaterr solu吐on”。．Thesesurveys
indicatedthat而也ishkelytobechan画n窑tooslowlyto
pr0Videenoug强，cha伯ctersforaphylogenedca11a1，sisin
thiscor加lex⋯o．Asa】1 indicat015ofnucjtotide
substitudonraCesuggesttllatmong而(，L，atpB，afld18S
rI)NAsequences．而cLismeleastconserved”川，intefest
血usexistsiIlfindin譬omerusefulDNAre2ionsmat
ev01vefastermandoes而fLtofacilitatether solu拈onof
phylogenetica伍nmesofthecewidiphyllaceae．
TheMfKgene，am turaseencondingge eloca【ed
基金项H：吲家杰出青年摹金(39825104)．[日家自然利节毖金
(30070053，40106014)广东眢自然科学基金r00("60)资助项日
·通汛联系人
作者箭介：章群(1968一)，男．博士．研究方向为丹f’|枣#
2003．05．02收藕．2003．05-20接受
万方数据
万方数据
2期 章群，等：连香树科及其近缘植物matk序列分析和系统学意义
witha lo“geVolunonarydistance，therelations}1ip
betweenT tracenn甚ceaeandC rcidiphyllaceaeisnotso
closeasIraditionaln℃atnlent．
Ljqujdanbar
J Un
Phn{ph_】】um
--__。一
B ．8】
FiglPhylogene吐ctreeb脚edon删fKgenesequeIKedatab，
the耻幢hbor0oiIIingmetbod．Numbersr印resentNJb00tstrap
val峭．ScaIebar；evoluⅡ哪ydistance∞lclllatedb，
Kjmu脚twop盯ametermetbod．
DaphniphyllaceaeThefh嘶lyisamonogeneric
familvwithlessthaIl40sDecies，a11res嘶cted“)eastem
Asia．butitssvstemadc"eatmentisvervconfused¨。．
Z蛔p^n咖^v““mhashadavaried上listory，alth叫ghpl ced
inHam蛐eli‘1aceaebvHamerasearlyas1904，waslong
thou￡hto be relatedto Buxaceaeand／or
EuphOrbiaceae”“．Morerecently， howeveLa
hamame】ideanamnitvhasbeenincreasinglvsupponed
bve“dencef}omwoodanatomy，stomatalypes，订oral
andfruitm曰即hology，palynology_ph”ochemistry，and
embryology”制．It has0nlvrarelybeenconsidered
cIoselvrelativeof Cercidiphyllaceae一’¨1．
HamarneIidaceousa衔nitywa 咖鲋ysuggestedby
manvc^dcalcharacterssharedbvthetwof删hes”⋯．A
consensusofcurren‘照onoIIlic叩inionfaVorsapos 石on
intheHamanlendae【l“．Ourphylogenencallalysisof
mⅡfKsequencesindicatedtllatCP"耐咖坶““Ⅲand
ZⅪp^n咖^vff加们fo珊aweaklysupponedcla e，whichis
al加Dstidenticalt0岫d。sequenceanalysisbyQiueta1．⋯1
ThecIosereladonships锄ongD即hniphy儿aceaend
CercidiphyllaceaeaI】dH ma lidaceaeareconfinnedby
o血ermoleculardata，althoughtheyareonlywea“y
supponc(1”。”1． As血eclade
D卵^yHfp^yf／“m．CP坩甜ip^ym删，wbichis thesister
grouptoHamamelidaceaeinourstudY，isju tweakly
supported(57％b00tsm甲)，andthebranchbetweenthe
cladea工1dHam枷eHdaceaeintIleNJ仃eeisshon(Fig．1)，
thephvlo譬ene石cr lationsMpsaInongme3familes8re
stjllnotresolved．
HhmameIidaceaeH Inamejidaceaeconsistof3I
窖enera，mainlydis缸butedin eastemAsia．The
phvlogene吐cimpljc撕onsofH锄锄elidaceaeisvery
contmversiaI．whichwillnotbe‘1iscussedh re．
AnentionsarejustpayedonmereIationshipsof
Ham锄elidaceaeandCercidiDhVllaceae．Assometlmes
thesubfnmiliesL岫uidambaroideaeandRhodoleioideae
arerecognizedasindependentta删1ies，(’uranalysis
compnses上j印“fd倒”6Ⅱr，尺^DdoZg妇andH“”mm￡f打【o
fepresentIhefa咖ly
Ah锄amelideana仟initvofCerc，diphvllaceaewas
行rscsuggescedbyBaiJlon(i87J)，whichw蛩sup一，fced
bvSolereder(1899)basedonmorphuIogy”。，a川has
sincereceivedsuppo九ffominvestigationsoI‘ seed
如atomy1fossjls，secondaryc mpounq曼1eafarchill’c【urc
andcDDNAresmcd【)nsj【evariation⋯。．Evidencefrom
蜥口fKseuuencesconnrmedtherelanonshiDsiH【he
presenceortheconsiderablysupponedcla ein～()lved
CercidiDhvllaceaeand HaInamelidaceaepJus
DaphniphvJlaceaeasnot dabove．Bootstrapsare}l谊her
foreachoftheselineagesin，H口rKLhanthatin，由rl。⋯o
In summary，the overantop()logv()f
Cercidlphyllaceae，D印hniphyllaceaeand
Ham锄elidaceaein ouranalvsisarein g‘lneral
agreementwitbthoseofpreviousmolecularanalyscs“”o．
Thus．severallinesofevidence。derivedftombo{hme
chlorop】astgenonleand18SrI)NAsequences．slj足譬esf
thepfesenceofconsidemblyphy ogenetica币n ties
betwcenCercidiphyllaceae，D phniphylllaceae，and
H锄amelidaceaeAlthou譬hbootstrapvaluesarehi曲er
f‘】r eachof￡hese1ineagesinmdrKthanin岫(1l，．【he
relationshi口s蛐on2血e3 far ili ssdllremainlobe
resolved．t王lismavbeattributabIein口a1【oextinctionof
manvllneagesin锄scomplexand／ortheweaksignalof
坍口水intllisgroup．WhiIe血isresultsisnotsⅢpris ng，
thea币nmesofCercidiphllaceaehasbeenprovidedwjth
higherbootstrapvaluesinm口rK【hanthosein小rL，l8S
a11datpB⋯1，indicating埘nfKhasa reatpo亡entlalin
resoIvin窖phvlogeniesatfamilylev landabove，alleast
amon2closelvrelatedfamiles．
Refbrences
【l】 DahlgrcnR1980AreVise‘lsystemofclassima(ion(1f【he
angjospems[J】肋，，L加如r，80：9】一124
【2】 EndressPK． 1986Floralstructure，8ystematics，and
phylogenyintmchodendrales[J]An”^缸5DH一厅fJf＆r，“73：
297—324．
【3】 cronquistA1981．An1nIegratedsystemofclass讯callunuf
nowe^ngPIantsfMJ NewY0rk：c州umb】a【nive一‘y
Press
【4】APG1998Anordinal cIassjncationforheranⅢlesof
nowe^ngplaIltslJ】A肌MlsJo“一B札Gd耐，84(4j53l一
553．
5】1酞ht目aIlA1996DiversityandclassincatjoIlofn0、veing
pla】1ts【M】．NewYork：c01umbiauniversityPress12814l
6】TangY1943Sys【emadc『la∞myo-thewoodsc，f Ihe
}Iamame】idaceae【J】肌盯Fhn^拈mDr池，mJf口fuf．】一63．
1bbles1—3
(71哂uYLchaseMWH00tsB 1998Phylogenc心s‘订the
HamaHlelidaeaIldthc打ames：parsimollya11ai?scs。f
nucleotides。quencesoftheplas6dgene岫cUJlm，，P胁Jl，
5cf，159(6)．1—13
下接第129页
万方数据
万方数据
连香树科及其近缘植物matK序列分析和系统学意义
作者： 章群， 聂湘平， 施苏华， 黄椰林， 谈凤笑， 张宏达
作者单位： 章群,聂湘平(暨南大学科学技术学院,广州,510632)， 施苏华,黄椰林,谈凤笑,张宏达(中山
大学科学学院,广州,510275)
刊名： 生态科学
英文刊名： ECOLOGIC SCIENCE
年，卷(期)： 2003,22(2)
被引用次数： 6次

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2.杜晓华.姚连芳 珍稀濒危植物-连香树研究进展[期刊论文]-河南科技学院学报（自然科学版） 2009(2)
3.刘志文.韩旭.李莉.李宪臻.陈温福 叶绿体和线粒体DNA在植物系统发育中的应用研究进展[期刊论文]-河南农业
科学 2008(7)
4.麦苗苗 连香树快速扩繁途径的研究[学位论文]硕士 2006
5.管玉霞 落叶松部分cpDNA、mtDNA及ITS序列研究及其应用于落叶松种的鉴定的可行性[学位论文]硕士 2006
6.章群 中药杜仲原植物的分子鉴定[期刊论文]-生态科学 2004(2)


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