Abstract:Mycelial total proteins in Hypsizygus marmoreus from Tris-saturated phenol isolation method was analyzed by two-dimensional electrophoresis(2-DE), then the 2-DE maps obtained were analyzed with software PDQuest8.0 to compare the protein expression difference between the optimum temperature(25℃)and high temperature stress conditions(42℃), and screen the high temperature stress-related proteins. The differential expression proteins were examined by matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS), and seven protein spots were identified. They are the Spa2 homology domain, DEHA2G15532p, peptidase M76 family, HSP70、SNF2 family of DNA repair proteins, cytochrome P450 and mixture of SAM domain family and zinc protein. The point 2 is up-regulated and the other six points were down-regulated. These high temperature stress-related proteins were involved in signal transduction, protein process, DNA repair and other general stress tolerance.