*"> <i>Narboh D</i>, a Respiratory Burst Oxidase Homolog in <i>Nicotiana attenuata</i>, is Required for Late Defense Responses After Herbivore Attack-文献传递-植物通论文库
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Narboh D, a Respiratory Burst Oxidase Homolog in Nicotiana attenuata, is Required for Late Defense Responses After Herbivore Attack


The superoxide (O2)-generating NADPH oxidases are crucial for the defense of plants against attack from pathogens; however, it remains unknown whether they also mediate responses against chewing insect herbivores. The transcripts of the respiratory burst NADPH oxidase homolog Narboh D in Nicotiana attenuate are rapidly and transiently elicited by wounding, and are amplified when Manduca sexta oral secretions (OS) are added to the wounds. The fatty-acid-amino-acid-conjugates (FACs), demonstrably the major elicitors in M. sexta OS, are responsible for the increase in Narboh D transcripts. Silencing Narboh D significantly reduced reactive oxygen species (ROS) levels after OS elicitation, but neither OS-elicited jasmonic acid (JA) or JA-isoleucine (JA-Ile) bursts, pivotal hormones that regulates plant resistance to herbivores, nor early transcripts of herbivore defense-related genes (NaJAR4 and NaPAL1), were influenced. However, late OS-elicited increases in trypsin proteinase inhibitors (TPIs), as well as the transcript levels of defense genes such as polyphenol oxidase, TPI and Thionin were significantly reduced. In addition, Narboh D-silenced plants were more vulnerable to insect herbivores, especially the larvae of the generalist Spodoptera littoralis. We thus conclude that Narboh D-based defenses play an important role in late herbivore-elicited responses.

Wu J, Wang L, W¨unsche H, Baldwin IT (2013) Narboh D, a respiratory burst oxidase homolog in Nicotiana attenuata, is required for late defense responses after herbivore attack. J. Integr. Plant Biol. 55(2), 187-198.