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作 者 ：刘志礼 李鹏云

期 刊 ：植物学通报 1998年 15卷 03期 页码：43-47

关键词：螺旋藻；盐胁迫；抗坏血酸过氧化物酶；过氧化氢酶；丙二醛；

摘 要 ：在0.1％～5.0％NaCl浓度范围的培养基中培养极大螺旋藻(Spirulina maxima)，发现NaCl浓度高于2.0％时螺旋藻生长受到明显抑制。培养7天后测定超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(ASA-POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量。结果表明：在盐胁迫下，SOD酶活性升高；抗坏血酸过氧化物酶和过氧化氢酶活性在低盐胁迫下活性升高，高盐胁迫下抗坏血酸过氧化物酶活性迅速降低，过氧化物酶则完全失活；MDA含量先随盐胁迫程度增加而降低，后随盐胁迫的进一步增强恢复至对照水平。

Abstract:Spirulina maxima was cultured in the media containing NaC1 concentration from 0.1% to 5.0%. It was obviously found that the growth of the algae was inhibited when NaC1 concentration was higher than 2.0%. Superoxide dismutase (SOD), ascorbate peroxidase (ASA-POD), catalase (CAT) and the content of MDA were determined after 7 days. The result showed that the activity of SOD increased under slat stress; the activity of ASA-POD and CAT increased under low stress,while the activity of ASA-POD decreased quickly and CAT was lost under higher stress; At first the content of MDA decreased when the salt stress was increasing,but then it came back to the level of the control with the increasment of the salt stress.

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