摘 要 ：利用RT_PCR方法从拟南芥（Arabidopsis thaliana (L.) Heynh.）中克隆了生长素结合蛋白（auxin binding protein 1）cDNA，进行了全序列测定。将该基因克隆在pBI121的35 S启动子和Nos终止子之间，得到植物表达载体p35EZ。通过根癌农杆菌（Agrobacterium tumefaciens (Smith et Townsend) Conn）介导的方法转化黄瓜(Cucumis sativus L.)。对转基因植株进行了PCR和Southern检测。所得到的转基因黄瓜植株单性结实能力增强。
Abstract:An auxin-binding protein 1 cDNA was isolated from Arabidopsis thaliana (L.) Heynh.by RT_PCR and confirmed by DNA sequencing. Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method. The intergration of the transgene was identified by PCR and Southern blot analysis. It
was found that the parthenocarpic ability of transgenic cucumber plants was enhanced.