摘 要 :本研究以四倍体大燕麦(Avena magna L.)做母本,六倍体裸燕麦(Avena nuda L.)做父本进行杂交,利用幼胚拯救技术获得了杂种F1,并对其后代形态特征进行了观察;对杂种F1同工酶图谱和DNA指纹图谱进行了分析。杂种F1形态特征偏亲本或介于双亲之间;同工酶研究表明多数F1具有双亲互补酶带;RAPD分析不同引物扩增产物F1呈共显性或偏父、偏母。这些结果表明F1为真杂种。
Abstract:The intergeneric hybrid between Avena magna and Avena nuda was successfully obtained by means of embryoes rescue. The hybrids F1 were intermediate between the parents in morphology, and their traits resembled either parents at different developmental stages. The zymogram pattern of hybrids F1 mainly displayed complementary pattern of the two parents. Genomic DNAs was used for RAPD analysis from parents and hybrids. Some primers show co-dominant or maternal-like or the some as male parent. The results indicated that the hybrid F1 we obtained is true hybrid.