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作 者 ：王明艳 孟丽军 张秀军

期 刊 ：植物学通报 2000年 17卷 05期 页码：470-472

关键词：质外体空间；树脂铸型技术；扫描电子显微镜(SEM)；

摘 要 ：本文介绍了用于研究植物体质外体空间三维结构的树脂铸型技术。植物包含许多重要的质外体空间，如木质部管状分子的腔隙、与气孔相连的叶肉细胞间的通气系统、分泌腔等等。这种空间的三维结构可借助于扫描电子显微镜(SEM)研究。但问题是，用SEM直接观察不到一个组织或细胞切面内部的图像，因此，不能观察它们的全貌。通过运用树脂铸型技术，可以获得完整的组织或腔隙内部空间的铸型。反映管壁结构的各种形象被印在铸型的表面上，在SEM下可对质外体空间进行详细研究。树脂铸型技术在结构植物学的研究上有重要的意义。

Abstract:A resin casting method for investigating apoplastic spaces of plant was intoouduced in this paper. A plant body contains a variety of important apoplastic spaces, such as the tracheary elements of xylem, the mesophyll‘s intercellular spaces that permit the diffusion of carbon dioxide away from stomata and secretory cavities. The study of the three_dimensional organization of such spaces has been greatly aided by scanning electron microscopy, But SEM can not be able to image internal part of a tissue or cell that are facing a cut surface, so samples are rarely observed in their entirety. By means of resin casting method, it should be possible to produce a lot of casts of tissue spaces and interal spaces of plant. Casts can be examined with SEM. Resin casting method is very important to the study of structural botany.

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