Two mutants possessing elongated uppermost internode, Xieqingzao eB-1 (XQZeB-1) and Xieqingzao eB-2 (XQZeB-2), were identified from M 2 population of Xieqingzao B-line (XQZB) treated with γ-ray. The proportion of uppermost internode length to entire culm length of XQZeB-2 and XQZeB-1 were 65.3%and 54.8%, respectively. Compared with the original XQZB, the increased length of uppermost internode of XQZeB-2 contributed to the total increased culm length by 90.2% as well as XQZeB-1 by 53.3%. Genetic analysis showed that the characters of elongated uppermost internode in the two mutants were governed by one pair of recessive gene respectively. The recessive gene of XQZeB-1 is allelic to the reported eui , but that of XQZeB-2 is non-allelic to it by allelic test. Therefore, the elongated-uppermost-internode gene of XQZeB-2 is a new gene, designated as eui2. Microsatellite markers RM258, RM269, RM271 and RM304, which were linked with eui2 and located on chromosome 10, were identified. The genetic distances from the four markers to eui2 were 12.0 cM, 12.9 cM, 35.1 cM, 1.4 cM, respectively. It could be concluded that eui2 gene was located on the middle of the long arm of chromosome 10.
水稻长穗颈高秆隐性基因eui2 的遗传及其微卫星分析
杨蜀岚1杨仁崔1* 曲雪萍2章清杞1 黄荣华1王斌2
（1福建农业大学作物遗传育种研究所，福州350002；2. 中国科学院遗传研究所，北京100101）
摘要： 从水稻 (Oryzasativa L .)协青早B的辐射M2 群体中获得以最上节间伸长为特征的长穗颈高秆突变系协青早eB-1和协青早eB-2 ,与原品系协青早B的秆性状相比 ,协青早eB-2的第一节间长占总秆长的 6 5 .3% ,其第一节间的增长量占秆长总增长量的 90 .2 % ;协青早eB-1的第一节间长占总秆长的 5 4.8% ,其第一节间的增长量占秆长总增长量的 5 3.3%。遗传分析表明 :协青早eB_1和协青早eB-2中的长穗颈高秆性状各由一对隐性基因控制 ,二者之间互不等位。与已报道的eui基因的等位性测验表明 :协青早eB_2的eui基因与其不等位 ,为新的长穗颈高秆隐性基因 ,命名为eui2 ;协青早eB-1的eui基因则与其等位。微卫星分析表明 :eui2基因与第 10染色体的RM2 5 8、RM2 6 9、RM2 71和RM30 4连锁 ,其遗传距离分别为 12 .0cM、12 .9cM、35 .1cM、1.4cM。由此推断 ,eui2基因位于第 10染色体长臂的中部
关键词： 水稻；eui；遗传；微卫星标记；杂交稻
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