Genetic diversity at Gli-1, Gli-2 and Glu-1 loci was investigated in 32 accessions of Chinese endemic wheat by using acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulfate (SDS)-PAGE. There were 8 gliadin and 3 high-molecular-weight (HMW)-glutenin patterns in 14 Yunnan hulled wheat ( Triticum aestivum ssp. yunnanese King) accessions, 9 gliadin and 4 HMW-glutenin patterns in 9 Tibetan weedrace ( T. aestivum ssp. tibetanum Shao ) accessions, and 9 gliadin and 5 HMW-glutenin patterns in 9 Xinjiang rice wheat ( T. petropavlovskyi Udacz. et Migusch.) accessions. One accession (i.e. Daomai 2) carried new subunits 2.1+10.1 encoded by Glu-D1 . Among the three Chinese endemic wheat groups, a total of 10, 14 and 11 alleles at Gli-1 locus; 11, 14 and 12 alleles at Gli-2 locus; and 5, 6 and 8 alleles at Glu-1 locus were identified, respectively. Among Yunnan hulled wheat, Tibetan weedrace and Xinjiang rice wheat, the Nei‘s genetic variation indexes were 0.3798, 0.5625 and 0.5693, respectively. These results suggested that Tibetan weedrace and Xinjiang rice wheat had higher genetic diversity than Yunnan hulled wheat.
中国特有小麦Gli-1、Gli-2 和Gli-1位点的遗传多样性
魏育明* 郑有良 周永红 刘登才 兰秀锦 颜泽洪 张志清
（四川农业大学小麦研究所，都江堰611830）
摘要：运用APAGE和SDS_PAGE方法,研究了32份中国特有小麦Gli-1、Gli-2和Glu-1位点的遗传多样性.在14份云南铁壳麦(Triticum aestivum ssp. yunnanese King)中,共出现8种醇溶蛋白带型和3种高分子谷蛋白带型.在9份西藏半野生小麦(T. aestivum ssp. tibetanum Shao )中,发现9种醇溶蛋白带型和4种高分子谷蛋白带型.在9份新疆稻麦(T. petropavlovskyi Udacz. et Migusch.)中,观察到9种醇溶蛋白带型和5种高分子谷蛋白带型,其中1份新疆稻麦(稻麦2)具有Glu-D1编码的新亚基2.1+10.1.在这3种中国特有小麦群体中,Gli-1位点分别检测出10、14和11个等位基因;Gli-2位点各具有11、14和12个等位基因;Glu_1位点也分别出现5、6和8个等位基因.云南铁壳麦、西藏半野生小麦和新疆稻麦群体内的Nei‘s遗传变异系数分别为0.3798、0.5625和0.5693.这些结果说明,与云南铁壳麦相比,西藏半野生小麦和新疆稻麦群体内的遗传变异相对较大.
关键词： 云南铁壳麦；西藏半野生小麦；新疆稻麦；遗传多样性；醇溶蛋白位点；高分子谷蛋白位点
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