A mRNA whole-mount in situ hybridization method is reported here for quick, direct analysis of the spatial and temporal mRNA expression patterns in plant young embryos. A cDNA clone THE3 (tobacco heart embryo 3) was isolated by differential screening from tobacco (Nicotiana tabacum L.) heart embryo cDNA library as compared with the globular embryo cDNA library. The distribution of THE3 mRNA in tobacco heart embryos and globular embryos was investigated by a whole-mount in situ hybridization technique, showing that THE 3 is preferentially expressed in heart embryos.
一种适于植物幼胚mRNA整体原位杂交的方法
李师弢 吕应堂 杨弘远*
（武汉大学生命科学学院发育生物学研究中心，武汉430072）
关键词： 幼胚；mRNA原位杂交；烟草
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