Abstract:By crossing bread wheat cultivar GC8901 with the 1D monosonic line of Xiaoyan No. 6 and backcrossing the offspring with the Xiaoyan No. 6 1D monosonic line for 5 years, high-molecular-weight glutenin subunits 1Dx5+1Dy10 from GC8901 have been transferred into wheat cultivar Xiaoyan No. 6. The BC5F1 offspring lines had been detected by using methods of cytology, marker, molecular marker and six elite single plants with high molecular-weight glutenin subunits: 1Ax1, 1Bx14+1By15, 1Dx5+1Dy10 were identified. Those lines have high-yield potential with better agronomic characters and have been used in high quality wheat breeding processes as well.（Authors for correspondence. E-mails: zzb@ms.sjziam.ac.cn and amzhang@genetics.ac.cn）