Suspension cell cultures of Maytenus hookeri Loes. (Celastraceae) in SH media were established from the calli induced from the leaves and young stems of M. hookeri on MS media with the supplement of 2 mg/L 2,4-D and 0.1 mg/L KIN (kinetin). Ethyl acetate extract of the cultures showed inhibitory activities against Penicillium avellaneum UC-4376 which was sensitive to maytansinoids. Exhaustive isolation of natural products from a large scale of suspension cell cultures did not yield maytansine instead of affording nine compounds including one novel triterp enoid, named 2,3-diacetoxyl maytenusone (1), and eight known ones including squalene (2), β-sitosterol (3), 2′,3′,4′-triacetyl-sitoindoside Ⅰ (4), salaspermic acid (5), maytenonic acid (6), 2α-hydroxy-maytenonic acid (7), 6,11,12-trihydroxy 8,11,13-abietrien-7-one (8) and 11,12 dihydroxy 8,11,13 abietatrien 7 one (9) elucidated on the basis of 1D and 2D-NMR data. The 1H-NMR and 13C-NMR assignments were made for 1, 5, 6 and 7, while the 13C-NMR assignments for 5 and 6 were revised. The chemical results suggested that the suspension cell cultures of M. hookeri did not produce maytansinoids under the reported experiment conditions.
云南美登木悬浮培养细胞的化学成分
鲁春华1 张建新2 甘烦远1 沈月毛1，2
（1. 中国科学院昆明植物研究所，昆明650204；2. 贵州省、中国科学院天然产物化学重点实验室，贵阳550002）
利用组织培养方法以云南美登木(Maytenus hookeri Lose.)未木质化的茎和嫩叶为材料诱导出愈伤组织,经过继代培养建立了悬浮细胞培养系.培养物的乙酸乙酯提取物显示抗橙色青霉(Penicillium avellaneum UC-4376)生长的生物活性.对培养物进行化学成分研究,分离鉴定了9个化合物:2,3-diacetoxyl maytenusone (1)、角鲨烯 (squalene,2)、β-谷甾醇 (β-sitosterol,3)、2′,3′,4′-triacetylsitoindoside Ⅰ (4)、salaspermic acid (5)、美登酮酸 (maytenonic acid,6)、2α-羟基美登酮酸(2α-hydroxy-maytenonic acid,7)、6,11,12-trihydroxy-8,11,13-abietrien-7-one (8)和11,12-dihydroxy-8,11,13-abietrien-7-one (9),其中化合物1为新化合物.通过2D NMR对化合物5-7的NMR数据进行了全指定,并修正了化合物5和6的部分碳谱数据指定.
关键词： 云南美登木；卫矛科；悬浮细胞培养；美登木素2，3-diacetoxyl maytenusone
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