Behavior of Meiotic Chromosomes in Pinus wallichiana Jacks, Pinus strobus L. and Their Hybrid and nrDNA Localization in Pollen Mother Cells of the Hybrid by Using FISH
作 者 ：Hui-Sheng Deng, Da-Ming Zhang, Cheng-Xin Fu and De-Yuan Hong
Abstract:The complete process of meiosis was investigated in Pinus wallichiana Jacks, Pinus strobus L. and their artificial hybrid (F1) using microsporocytes. It is revealed that there were slightly lower chiasma frequency, lower ring bivalent frequency, lower meiotic index and distinctly higher frequency of aberrance (chromosomal bridges, fragments or micronuclei) in pollen mother cells (PMCs) of the hybrid (F1) than those of the parental species, which show a certain degree of differentiation between homologous chromosomes of the two parents. However, relatively higher frequency of ring bivalents and higher meiotic index in all the three entities indicate the great stability of genomes of parental species, and the differentiation of genomes between the two parents must have been slight. Total nineteen signal loci of 18S rDNA were observed in nine bivalents of the hybrid (F1), among which one bivalent bears two loci, while the others have only one. It is suggested that distinct differentiation at genetic level existed in homologous chromosomes of the two parental species, whereas only slight differentiation at karyotypic and genomic levels take place between the parent species.