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Characterization of a S-locus-related Receptor-like Kinase Cluster in Rice Chromosome 4


We have identified 14 S locus glycoprotein (SLG) related protein kinase genes in a 323 kb contig of rice (Oryza sativa L.) chromosome 4 and we detected the transcription pattern of this gene cluster by reverse transcription polymerase reaction (RT-PCR). RT PCR results revealed that nine putative genes were transcribed in rice and these genes had the different expression patterns: two genes are expressed predominantly in reproductive tissues while the other seven genes are expressed in both reproductive and vegetative tissues. Analysis of the predicted amino acid sequences demonstrated that the extracellular receptor domains are highly homologous to SLG of Brassica, whereas the cytoplasmic kinase domains contain conserved amino acids present in serine/threonine kinases.

对水稻4号染色体一个编码S位点相关的受体样蛋白激酶基因簇的分析
雷海燕1,2 周波1,3*  洪国藩1,2**韩 斌6**

(1. 中国科学院上海生命科学研究院国家基因研究中心,上海>200233;
2. 中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031;
3. Department of Plant Pathology, Ohio State University,Columbus,Ohio,43210,USA)

摘要: 通过对水稻(Oryza sativa L.) 4号染色体一段323 kb 的序列测定和分析,在其中108 kb的区域内发现了一个由14个编码S位点相关的受体样蛋白激酶(SRK)基因组成的基因簇.RT-PCR实验证明了这14个基因中有9个基因表达,并且这9个基因有不同的表达模式: 其中2个基因主要在生殖器官中表达, 而另外7个基因在水稻的营养和生殖器官中均有表达.对这些基因的预测的氨基酸序列进行分析表明他们的细胞外受体部分均和甘蓝的SLG蛋白高度同源,而细胞内的激酶区都包含有丝氨酸/苏氨酸激酶中特异的氨基酸.

关键词: 水稻受体样激酶蛋白;S位点受体激酶;S位点糖蛋白;水稻

通讯作者。Fax.+86(0)21 64825775. 韩斌,Tel:+86 (0)21 64848560;E-mail: <bhan @ newnetra.ncgr.ac.cn>.洪国藩,Tel:+86 (0 )21 64822885;E-mail: <gfhong @ enwnetra.ncgr.ac.cn>


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