Oryza sativa L. ssp. japonica and indica exhibit different sensitivity to photoinhibition and they show different stability of their core proteins D1 in the chloroplast photosystem Ⅱ. Using in situ hybridization, psbA , the gene encoding D1 protein of O. sativa ssp. japonica cv. 9516, and that of O. sativa ssp. indica cv. Shanyou 63 was cloned. As revealed by homology comparison of their sequences, the sequences are identical in the regions of promoter and 5′-UTR; differences are found in individual bases in the coding region all of which, being in the third position of respective codons, however, do not affect the amino acids coded finally; a difference is noted in the length of the oligo-U sequence in the region of 3′-UTR. It is thus apparent that, rather than a result of any difference in the amino acid sequences, the differences in the sensitivity to photoinhibition of D1 proteins between japonica and indica rice may be related to the upstream factors that regulate expression of psbA or to differences of photoprotective mechanisms.
粳稻和籼稻D1蛋白光抑制敏感性的差异与其编码基因序列结构特征的相关性分析
张方 谢先芝 陈凡 吴乃虎*
（中国科学院发育生物学研究所，北京100080）
摘要：粳稻(Oryza sativa L. ssp. japonica)和籼稻(O.sativa ssp. indica)对光抑制的敏感程度存在差异,它们的叶绿体光反应中心Ⅱ核心蛋白D1的稳定性不同.以菌落原位杂交法克隆了粳稻"9516”和籼稻"籼优63”叶绿体 D1蛋白的编码基因psbA.核苷酸序列同源比较显示:两者在启动子区和5′-UTR完全相同;编码区存在着个别碱基的差异,但均位于三联体密码的第三位,不影响氨基酸编码特性 ,在蛋白质氨基酸序列上没有差异;在3′-UTR内存在寡聚U长度的差异.因此,粳稻和籼稻 D1 蛋白对光抑制作用敏感性的差异与其蛋白质的氨基酸序列结构无关,可能与调控psbA基因表达的上游因子或光保护机制的差异有关.
关键词： 粳稻；籼稻；光抑制；D1 蛋白；psbA
全 文 ：