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作 者 ：ZHENG Zhen Gui*, ZHOU Yu, LIU Di, HU Zhi Bi

Keywords:full habituated cells, ajmalicine, cell culture, Catharanthus roseus,

Abstract:

A full habituated cell line C20 hi was screened from 2,4 D dependent line (C20D) of Catharanthus roseus (L.) G. Don. The investigation involved the cell growth, ajmalicine production and enzyme activity related to indole alkaloid biosynthesisin both cell lines. These results indicated that C20 hi cells grew faster than C20D cells, and average ajmalicine content in C20 hi cells was 18.4 times more than that in C20D when cultured in the production medium. In the growth medium, average aj malicine content in C20hi cells was 31.9 times more than that in C20D cells, while the cell growth has no obvious difference. The comparison of enzyme activities in C20hi and C20D cells indicated that tryptophan decarboxylase (TDC),strictosidine synthase (SSS) and geraniol 10 dehydrogenase (G10H) activities have no close relation to ajmalicine accumulation, although the activities of these enzymes were higher when cells were cultured in the production medium than in the growth medium. The C20hi cells are relatively stable in five years of culture.

长春花激素完全适应型细胞的生长和阿玛碱合成特性
郑珍贵* 周 煜 刘 涤 胡之璧
（上海中医药大学中药研究所，上海200032）

摘要： 从长春花激素依赖型细胞系 (C_{2 0}D)筛选出一种激素完全适应型的细胞系 (C_{2 0} hi) ,考察了两种细胞生长、阿玛碱合成和与吲哚生物碱生物合成相关的酶的活性 ,结果表明 :在生长培养基上二者生长无显著差异 ,而C_{2 0} hi细胞平均阿玛碱含量是C_{2 0} D的 31 9倍 ,在生产培养基上C_{2 0} hi细胞生长较C_{2 0} D快 ,C_{2 0} hi平均阿玛碱含量是C_{2 0} D的1 8 4倍。通过比较生产和生长培养基中C_{2 0} hi细胞的色氨酸脱羧酶、异胡豆苷合酶和牛儿醇-1 0-脱氢酶活性 ,发现在生产培养基中培养细胞的 3种酶活性均显著高于生长培养基 ,但与阿玛碱积累没有密切关系。研究结果还表明 ,通过 5年的继代培养 ,激素完全适应型细胞系C_{2 0} hi的阿玛碱含量是比较稳定的。
关键词： 完全适应型细胞；阿玛碱；细胞培养；长春花

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