The genetic diversity among 32 accessions ofHordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS-PCR primer sets derived from RFLP clones of the wheat (Triticum aestivum L.) or barley (Hordeum vulgare L.) mapping. Out of the 22 STS-PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with HinfⅠ, HhaⅠ, HaeⅢ and RsaⅠ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32H. bogdanii accessions, a total of 315 bands were observed in 88 STS-PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS-PCR based genetic diversity index (GD) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the geneticrelationships between accessions was constructed using the unweighted pair group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguish hed by STS-PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.
应用STS-PCR 标记研究新疆布顿大麦的遗传多样性
郭 红1,2 魏育明2* 陈 放1 郑有良2*
（1. 四川大学生命科学学院，成都610064；2. 四川农业大学小麦研究所，都江堰611830）
摘要： 利用22个来源于小麦(Triticum aestivum L.)和栽培大麦(Hordeum vulgare L.)的STS-PCR标记,研究了32份新疆布顿大麦(Hordeum bogdanii Wilensky)的遗传多样性.在这22个STS-PCR标记中,仅有3个标记的扩增产物经HinfⅠ、HhaⅠ、HaeⅢ和RsaⅠ 4种限制性内切酶消化后没有产生多态性DNA片段,而19个标记(占86.4%)和46种标记/酶组合(占52.3%)能够揭示材料间的多态性.在32份布顿大麦材料的88种STS-PCR标记/酶组合中,总共得到315条DNA片段,平均每个标记/酶组合能得到3.6条DNA片段.在这315条DNA片段中,共有123条片段(占39.0%)具有多态性,每一个多态性标记/酶组合能获得1～6条多态性DNA片段.STS-PCR标记揭示的32份布顿大麦的遗传距离变化范围为0.078～0.352,平均为0.198.根据STS-PCR标记的遗传距离矩阵,采用不完全加权算术平均数法(UPGMA)构建了32份布顿大麦群体间的遗传关系树状图,结果表明STS-PCR标记能将32份材料完全区分开来.同时,来源于同一地方的不同居群没有明显地聚类在一起,表明新疆布顿大麦的遗传多样性与其地理分布相关不紧密.
关键词： 布顿大麦；STS-PCR 标记；遗传多样性；遗传关系
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