Abstract:From the root of Thalictrum acutifolium (Hand. -Mazz.) Boivin eight chemical constitions, seven of them ware identified as methylp UV, MS, 1H and 13C-NMR) and chemical reactions, seven of them were identified as methylpalmitate, cis-9-cis-12-methyloctadecadienoate, β-sitosterol, nonacosane, n-pentatriacontane, oxyberberine and trilobinine (1,11-dihydroxy-2,9- dimethoxy-6-dimethyl-aporphine). Acutifolidine is a new compound. Its structure was deduced to be 2-hydroxy-1, 8, 9, 10-tetramethoxy-6-methyl-aporphine. Trilobinine was first isolated from genus Thelictrum.