Abstract:A non-segregating F2 population was obtained from the cross of tfiploid × diploid in rice ( Oryza sativa L. ). The maternal parent used in cross was SAR-3 which is an autotriploid occurring in the field, and the paternal was a restored line Sheng 47. The F2 population from the same F1 plant appears uniform, and so does F3. Results from coefficients of variation (CV) of F2, Ducan’s test of F3 and SSRs marker analysis showed that the F2 of SAR-3 × Sheng 47 was non-segregating. Markers for either parent could be present in F2 progenies. In some cases, both markers for each parent could be found on one chromosome. The authors presume that after crossing there might be recombination of chromosome followed by homozygotization. Stability of early generation could be utilized in breeding, as it is a new approach to shorten the breeding procedures.