Abstract:Callus colony PL1 possessing the potential for a high frequency of plant regeneration (997.1%) was obtained from selected brown and green calli of Astragalus grubovii explants in subcultures. A relatively homogeneous cell suspension line was established from the callus colony in liquid Bx medium containing NO3- and NH4+ at a NO3-/NH4+ of 10:1 on a rotary shaker. Calli having a high frequency of plant regeneration (968.9%) were obtained from the the cell suspension line in monocell cultures.