Abstract:Two steroidal glycosides were isolated from the rhizome of Aspidistra leshanensis K. Y. Lang et Z. Y. Zhu. Their structures were established as 5-O-β-D-glucopyranosyl-convalla- genin-B and aspidistrin on the basis of chemical and spectral evidences. Diosgenin, isonarthogenin, convallagenin-B, β-sitosterol and a sapogenin with three hydroxyls were obtained from the acid-treated n-BuOH extract.