Abstract:Phosphomannomutase (PMM) was isolated and further purified from the fresh seeds of Sesbania cannabina by using preparative Sephadex G-200 slab IEF. The pH of Sesbania PMM was 5.0. Purified PMM showed a distinct band on SDS-PAGE with the molecular weight of 41 kD. The pH value for optimum catalysis of the enzyme was 7.3 at 30℃, and the equilibrium constant of PMM reaction was 16.2. In addition, the amino acid composition of the purified PMM was measured which conformed the property of acidic protein.