Abstract:A protein accumulation has been observed in carrot cell line L104-2 after adapting to grow in Murashige and Skoog medium supplemented with 1% NaCl. The apparent molecular weight of its monomer was estimated at about 66 kD by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Gathered together with the fact that other osmotic reagents such as polyethyleneglycol did not induce its accumulation which indicated that this 66 kD protein is somewhat different from the 26 kD osmotins reported before from tobacco and tomato cells, therefore, it seems a salt adaptation protein.