Abstract:Six steroid compounds were isolated from the ethereal soluble fraction of the spores of Ganoderma lucidum. Based on their chemical properties and MS, UV, IR, 1H NMR and 13C NMR spectral analysis, they were identified as ergosta-7,22-dien-3β,5α, 6β-triol; ergosta- 7, 22-dien-3β,5α, 6α-triol; ergosta-7, 9, 22-trien-3β, 5α, 6α-triol; ergosterol palmitare; ergosta-4, 6, 8(14), 22-tetraen-3-one and ergosterol respectively.