Abstract:Four secoiridoid glycosides were isolated from Swertia nervosa (G. Don) Wall. ex C.B. Clarke. Three of them were identified as vegeloside (Ⅰ), sweroside (Ⅲ) and swertiamarin (Ⅳ)on the basis of spectral and chemical evidences. Another compound, nervoside (Ⅱ), was a new secoiridoid glycoside, its structure was elucidated by means of UV, IR, 1H and 13C-NMR spectra. Nervoside was a 7-α-OCH3 isomer of vegeloside, this was confirmed by NOE experiment.