Cloning and Sequencing of a cDNA Encoding Farnesyl Pyrophosphate Synthase from Gossypium arboreum and Its Expression Pattern in the Developing Seeds of Gossypium hirsutum cv.
Abstract:A cDNA encoding farnesyl pyrophosphate (FPP) synthase was isolated from Gossypium arboreum L. cDNA library by using nest PCR and PCR-96 well plate screening methods. Nucleotide sequencing revealed that it is a full-length cDNA of 1.28 kb, and the deduced peptide contained 342 amino acids, the putative amino acid sequence exhibited 80.7%, 78.9% and 71.6% identities with the FPP synthases of Artemisia annua, Arabidopsis thaliana and Zea mays, respectively. Quantitative RT-PCR analyzed the steady-state level of fpsl mRNA in the developing seeds of G. hirsutum L. cv. "Surnian-6", the results revealed that the mRNA level was relatively stable in the early stages of the developing seeds, however, from 27 days post anthesis (DPA) to 40 DPA, transcription of fpsl mRNA was profoundly upregulated, the increment of the fpsl mRNA relative content was in combination with a concomitant accumulation of sesquiterpene aldehydes in the developing seed at this stage.