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Microdissection and PCR Amplification of Rye Chromosome

作 者 ：SONG Wen-Qin, LI Xiu-Lan, XU Wen-Sheng and CHEN Rui-Yang

Keywords:Chromosome of rye, Microdissection, PCR amplification，,

Abstract:The 18 ( 14A + 4B) chromosomes of rye ( Secale cereale L. ) were isolated by microdissection technique and the chromosomal DNA was amplified using the artificial synthetic single unique oligonucleotide-primed polymerase chain reaction (SUP-PCR). Southern hybridization demonstrated that the SUP-PCR products have homology with rye genome DNA.

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