Abstract:The nuclear matrix (NM) of pollen mother cells (PMC) of Lilium davidii var. willmottiae (Wilson) Roffill was studied using selective extraction, embeddment-free electron microscopy and whole mount cell preparation. A complex internal substructure, which is similar to the nuclear matrix in vertebrate cells, was observed in the nucleus of PMCs at prophase I . These nuclear matrices, comprising delicate protein filaments, spreaded over the nuclear region. The diameters of NM filaments were in the range of 5 - 15 nm. Nueleoli and chromosomes were found to be connected with the NM filament network. The NM was proposed to offer a framework for the topological change of chromatin/chromosome, and might be involved in the construction of the scaffold of metophase chromosome.