Abstract:The leaf esterase(ESTL), endosperm esterase(ESTE) and coleoptile esterase(ESTC) zymogram phenotypes in Set I wheat-wheatgrass alien additional lines and their parents were analysed. The wheatgrass genes Este-Agi1 and Estc-Agil were located onto the wheatgrass chromosomes of line TAI-12, the gene Este-Agi3 onto TAI-14; and the gene Este-Agi4 onto TAI-15.On the basis of some plant morphologies as well as those gene locations it might be deduced that TAI-12, TAI-14 and TAI-15 carry the wheatgrass chromosomes homoelogous to the wheat chromosomes of homoeologous groups 3, 7 and 6, respectively.