Abstract:PS Ⅱcore antenna complexes, CP43 and CP47, were purified from spinach (Spinacia oleracea L. ) by DEAE-Fractogel rISK 650S anion-exchange chromatography. Their normal temperature (298 K) resonance Raman spectra were measured. The results suggest that all β-carotenoids bound to CP43 and CP47 are in all-trans configuration and likely in twisted conformations.