Abstract:Seven crystalline constituents were obtained from acidic alcoholic extract of root of Euphorbia ebracteolata. They were identified as octacosanoic acid, daucosterol, β-sitosterol, bis (5-formyl-furfuryl)-ether (Ⅳ), a triterpenic acid, 2,4-dihydroxy-6-methoxyl-3-methyl-acetoph-enone (Ⅵ) and 2-β-glucoside of (Ⅵ) respectively. Three of them (Ⅳ, Ⅴ and Ⅵ) revealed inhibitory effect against tuberculous bacillus..In this paper, the authors reported identification of compound Ⅳ which was found in this plant first time. Its extract has a remarkable effect for the treatment of tuberculosis.