Abstract:Five alkaloids were isolated from the whole plants of Corydalis hendersonii Hemsl (= C. nepalensis Kitamura) by means of centrifugal TLC method. Alkaloids Cn-20(I) was assigned as a new alkaloid named henderine. Alkaloids Cn-1, Cn-2, Ch-16 and Cn- 19 were identified as β-allocryptopine, protopine, stylopine and Cheilanthifoline, respectively, based on mp. UV. IR. MS and 1H-NMR spectra.