Abstract:The triglycerides of fats from several varieties of Sapium sebiferum (L.) Roxb. were determined by means of pancreatic lipase hydrolysis technigue. Triglycerides of the fats. are composed of 9–14 kinds of triglycerides. They are similar to Cocoa butter. Fatty acid composition in 2-position of glycerides is oleic acid in more than 82%. The disaturated glycerides account for 81-88%, in which POP is in the majority (74–81%); the proportion of monosaturated glycerides is 1.8–6.0%; the trisaturated glycerides hold 9.6–15.6%, in which the main one is PPP (8.5–14.0%). The fats can be used for making Cocoa butter substitute by proper processing. Fat contents of the four varieties (“Da Li Ji Zhao”, “Wu Gong”, “Tong Chui” and “Xiao Li Ji Zhao”) cultivated in Guangxi province are richer (32.0–34.3%) than that of the wild species of the same plant. The proportion of disaturated glycerides is the highest (88.0%) and that of trisaturated glycerides is the lowest (9.6%) in fat from "Da Li Ji Zhao".