作 者 ：HONG Ya-Ping, CHEN Zhi-Duan, LU An-Ming
期 刊 ：植物分类学报 2001年 2期
Keywords:Menispeermaceae, trib．Menispermeae, Internal transcribed spacers(ITS), Phylogeny,
Abstract:
The phylogeny of the tribe Menispermeae (Menispermaceae) represented by 20 species
of 9 genera in China, was reconstructed based on sequence analysis of the internal transcribed spac-
ers (ITS) (including ITS1, ITS2, and 5.8S rRNA gene ) of nuclear ribosomal DNA. Three species
of two genera in the tribe Tinosporeae were designated as outgroups. Direct PCR sequencing method
was used in the study, The sizes of ITS within trib. Menispermeae range from 527 to 601 bp. The
aligned length is 667 bp, which provides 281 phylogenetically informative sites when gaps are treat-
ed as missing. The results of phylogenetic analyses show that: ① trib. Menispermeae is a monophyl-
etic group strongly supported by a bootstrap value of 100%; ② Pachygone valida, whose systematic
position was uncertain in the previous classification, should be placed in the Cocculus. ③Sinomeni-
um and Menispermum are two close genera of the tribe. Their sequcences are very similar to each
other, with ITS1 having 41 to 73 bp longer than that of the other genera in trib. Menispermeae. ④
Stephania and Cyclea are also closely related. The former forms two major clades, which are ap-
proximately consistent with the two traditional subgenera: subgen. Stephania and subgen. Tuberiph-
ania. The species of Cyclea are mutually little diverged in complete ITS sequences, and they com-
prise a sister clade to the genus Stephania.