全 文 ：Chinese Bulletin of Life Sciences
17 2
2005 4
Vol. 17, No. 2
Apr., 2005
315100
-
; ; ; ;
Q943.2; S432.1 A
Marker-assisted selection molecular mapping and cloning
of the rice blast resistance genes
WANG Zhong-Hua*
(Institute of Biotechnology, Zhejiang Wanli University, Ningbo 315100, China)
Abstract: The interaction between the rice resistance(R) genes and the AVR genes of fungal pathogen
Magnaporthe grisea is one of the model systems of the researches on plant molecular pathology and disease
resistance breeding worldwide. Molecular mapping, cloning and marker-assisted selection of the rice R genes
are the important field in this system. The paper briefly reviewed the progress of studies on this aspect to
provide the interest information for rice disease resistance breeding.
Key words: rice (Oryza sativa L.); the blast resistance genes; marker-assisted selection; molecular mapping;
cloning
[1]
1975~1990 16
1.57 [2]
20 90
380
[ 3 ]
2004-06-14 2004-09-10
( 1 9 7 2 ) *
1004-0374(2005)02-0183-06
20 60
30
[4]
184
DNA
( m a p p e d - b a s e d
cloning) Pi-b Pi-ta[5~6]
1
(marker-assisted selection, MAS)
Huang [7]
MAS F4 4 Xa-4 Xa-5
Xa-13 Xa-21 IRBB60 Singh [8]
MAS 3 Xa-5 Xa-13
Xa-21 PR106 Hittalmani [9]
MAS Pi-1 Pi-z5 Pi-ta
BL124
MAS 3 3
MAS
[10] MAS
243
PCR 46
43 93.5%
[11]
Pi-d(t) RM262
8987
F2 MAS
98% Chen [12] MAS
Xa-21
63 Xa-21 1kb
Xa-21 63
[13] MAS
Xa-21
R8006 R1176
[14]
3418S
MAS
Pi-ta 12
928
[6] Jia [15]
Pi-ta
pi-ta
Pi-ta
pi-ta DNA
YL100/YL102 YL153/YL154 YL155/YL87
YL200/YL87
Pi-ta
pi-ta
PCR
DNA GenBank
DNA [16~17]
F3 350 118
Pi-ta [18]
2
2.1
20
(restriction fragment length polymorphism RFLP)
RFLP DNA
cDNA cDNA
1988 McCouch [19] RFLP
F2
135
1 389 cM Causse [20]
726
1 491 cM Kurata [21]
1 383 RFLP 1 575
cM 1.14 cM F2
DNA
RFLP
( BC
RIL NIL DH ) RFLP
RFLP
RFLP
(polymerase chain reaction
PCR) PCR
DNA(random ampli-
fied polymorphic DNA RAPD)
(amplified fragment length polymorphism AFLP)
(simple sequence length polymorphism
SSLP) (random amplified micro-
satillite polymorphism RAMP) (sequence-
tagged sites, STS) DNA (DNA amplified
fingerprinting, DAF) (amplicon length
polymorphism, ALP) (specific ampilicon
polymorphism, SAP) (single strand con-
formation polymorphism SSCP) (single
nucleotide polymorphisms, SNPs)
(resistance gene analog, RGA) [22~23]
RFLP
PCR
2.2
DNA
30 1
Yu [24~25]
Pi-1(t) [Pi-
1] Pi-2(t) [Pi-z5] Pi-4(t)[Pi-ta] 11
6 12
14.0 2.8 15.3 cM Wang [26]
CO39/Moroberekan
Pi-5(t) Pi-7(t) 4
RFLP RG498 RG788 11
RG16 RG103A
10 QTL QTL
8
Naqvi [27] RAPD RFLP
Pi-10(t)
5 [28]
RAPD 8
Pi-zh[Pi-11(t)] 8
RAPD BP127A 14.9cM
8
[29] RFLP
Pi-h-1(t)
12 RFLP RG869 5.1 cM
Miyamoto [30]
Pi-b Pi-b 2
RFLP RZ123 RAPD
b-1 RZ213
G1234 RAPD b-1
1.9 cM RAPD b-1
0.5 cM Pi-b
Wang 1999
Rybka [31] RFLP
RAPD Tadukan
Pi-ta Pi-ta2 12
XNpb289 XNpb196
RAPD ta2-1 (0.3 cM) RFLP
XNpb088 (0.7 cM) Wu [32]
1 440 RAPD 18
Pi- ta
3 Pi-ta
186
1
Pi-1 LAC23 11 RZ536 7.9 [9]
Pi-1(t) Npb181 3.5
Pi-z Niigatawase 6 P130 1.6 [38]
R10069s 1.3
Pi-k Lebonnet 11 MRG233 2.1 [39]
RM144 3.6
Pi-2(t) 5173 6 RZ612 7.2 [9]
R2123 2.2 [40]
Pi-z5 RG64 0.9 [41]
AP22 1.2
SSR140 0.9 [42]
JSH12 0.9
Pi-ta2 Tadukan 12 XNpb088 0.7 [31]
RUBss 0.3
Pi-5(t) Moroberekan 4 RG498 < 12.0 [26]
Pi-6(t) Apura 12 RG869 15.4 [25]
Pi-7(t) Moroberekan 11 RG103A <16.0 [26]
R251 5.3
C30662 5.3 [43]
Pi-9(t) 6 PBA14 0.2 [40]
RG64 2.2
R2123 2.2
Pi-10(t) Tongil 5 RRF6 3.8 [27]
RRH18 2.9
Pi-11(t) 8 8 Bp127A 14.9 [28]
Pi-zh RG181B 20.7
Pi-h-1(t) 12 RG869 5.1 [29]
Pi-15 GA25 9 BAPi15486 0.35 [44]
BAPi15782 0.35
BAPi15844 1.1
Pi-18(t) Suweon 365 11 RZ536 5.4 [45]
Pi-20(t) IR24 12 XNpb4.2 14.0 [46]
XNpb88 1.0
pi-21 Owarihatamochi 4 G271 5.0 [47]
G317 8.5
Pi-24(t) Chubu 32 11 C1172 4.8 [48]
E2021 11.7
156 12 OPH11400 1.0 [49]
OPD10800 1.0
Pi-25(t) 2 6 K171400 2.4 [49]
A7500 7.5
Pi-26(t) 2 6 RGA24a 4.9 [50]
RGA6a 8.2
Pi-29(t) IR64 8 G104 6.5 [51]
Pi-33(t) IR64 8 Y2643L 0.9 [52]
Bala G1010 3.6
Pi-44(t) Moroberekan 11 AF348 11.0 [53]
AF349 3.3
Pi-CO39 CO39 11 S2712 1.0 [54]
Pi-d(t) 2 RM262 6.9 [55]
RPR1 Asominori 11 RM120 3.8 [56]
Pb1 Modan 11 S723 1.2 [57]
0.5 cM Pi-ta
Bryan 2000
Pi-b 1 251
(nucleotide binding site, NBS)
(leucine-rich repeats, LRR)
N NBS 1a 2 3a
LRR 8 [5]
Pi-b
Pi-ta
928 LRR
[6]
Jia [33] Pi-ta
AVR-Pita
Pi-2(t) Pi-9(t)
Leong AVR1-CO39/
P i - C O 3 9 ( t )
Ronald Pi-3 Pi-5
Kawasaki
Pi-ta2 Pi-2(t)
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