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作 者 ：朱玮阁,郭瑞昕,杨家新*

期 刊 ：生态学报 2009年 29卷 7期 页码：3605~3612

关键词：

Keywords:fenvalerate, TBTC, Brachionus calyciflorus, batch carrying eggs, mictic female production,

摘 要 ：25℃，投喂密度为(2~3)×106cells/ml的蛋白核小球藻，采用单“克隆”培养的方法对萼花臂尾轮虫（Brachionus calyciflorus）进行慢性毒性实验，分别比较具有环境雌性和雄性激素效应的药物氰戊菊酯(Fenvalerate)和三丁基氯化锡(TBTC)对萼花臂尾轮虫亲代单次携卵量、总产卵量和后代数、以及亲代和F1代的混交比率的影响。结果表明：氰戊菊酯处理组，亲代单次携2和3个卵的频次在浓度达400μg/L时都显著高于对照组（p<0.05），而亲代单次携1个卵的频次都显著低于对照组（p<0.05）。各浓度组总产卵量都显著高于对照组（p<0.05），后代数也明显增高。氰戊菊酯在两个最低实验浓度（0.64μg/L和3.2μg/L）就会对亲代混交率和子一代混交率、MF/AF存在显著影响。TBTC处理组在浓度00016、0.008μg/L和1μg/L亲代单次携1个卵的频次显著高于对照组，而携3个和4个卵的频次显著低于对照组（p<0.01）。亲代总产卵量和所产后代数在TBTC各处理组都显著低于对照组（0.0016μg/L除外）（p<0.05）。而子一代的混交率、MF/AF等都显著低于对照组（p<005）。实验表明氰戊菊酯和TBTC对萼花臂尾轮虫的生殖具有明显的内分泌干扰效应。

Abstract:Endocrine disruptors (EDs) are a structurally diverse group of compounds that may adversely affect the health of humans, wildlife and fisheries, or their progeny by interaction with the endocrine system. Many EDs have been detected in aquatic environments, many of which are capable of disrupting endocrine functions of a variety of aquatic invertebrates. In the present study, the estrogenic compound Fenvalerate and androgenic compound TBTC were screened for their effect on the batch carrying eggs every parental rotifer (P)，total numbers of eggs, offspring production, and mictic female production of the freshwater rotifer Brachionus calyciflorus. Treatment with Fenvalerate at concentrations (except 3.2μg/L) significantly increased the frequency of carrying 3 eggs (p<0.05).Treatments with Fenvalerate at concentrations of 32μg/L to 2000μg/L significantly increased the total number of eggs production compared to controls (p<0.05). Treatment with TBTC at 0.0016，0.04, 0.2 and 1μg/L resulted in a significantly higher frequency of females carrying 3 and 4 eggs compared to control (p<0.05), but the frequency of carrying 1 egg was lower than control. The total number of eggs and offspring were all significantly lower than control (0.0016μg/L excluded) (p<0.05). Moreover, the ratios of mictic female (MF%) of parents and the first generation (F1) were also affected by Fenvalerate and TBTC. MF% of parents and F1 was significantly lower than control only in the two lowest concentrations of Fenvalerate at 0.64μg/L and 32μg/L (p<0.01), but there was no difference between treatments and control in higher concentrations, and no effect on MF/AF (mictic females/amictic females) of the F1 as well. The concentrations of TBTC only over 5μg/L significantly affected the MF% of parental compared to control (p<0.01), but MF% of F1 was obviously affected at all concentrations except 02μg/L. The MF/AF of parents and F1 were quite similar. TBTC had a negative effect on total number of eggs and offspring, whereas Fenvalerate increased them. Consequently, we can conclude that Fenvalerate and TBTC significantly affect the reproduction of rotifers through mechanisms that are not well understood.

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