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作 者 ：刘铸,田秀华,白素英

期 刊 ：生态学报 2007年 27卷 6期 页码：2435~2442

关键词：大鸨东方亚种；大鸨指名亚种；遗传多样性；系统分化；

Keywords:Otis tarda dybowskii, Otis tarda tarda, genetic diversity, phylogenetic differentiation,

摘 要 ：大鸨(Otis tarda)为中国Ⅰ级重点保护动物，分为两个亚种，即指名亚种(Otis tarda tarda)和东方亚种(Otis tarda dybowskii)。研究从代表母系遗传特征的mtDNA控制区和代表双亲遗传特征的核微卫星两方面对两个亚种的遗传多样性与系统分化进行了分析。指名亚种mtDNA控制区3段序列(CtrIaL/CtrIIoH、L438/H772和LCR2a/HCR8)的单倍型数、Л、δ和К都明显高于东方亚种，更显著高于松辽平原西北部繁殖区。东方亚种3个微卫星(Otmic08、Otmic16和Otmic26)的等位基因数、Ho和He明显低于指名亚种。因此东方亚种的遗传多样性都明显低于指名亚种，甚至低于Madrid种群。两个亚种存在于不同的系统分支，证实了两个亚种的系统关系，欧洲指名亚种存在更多的系统分支。

Abstract:Otis tarda is listed as a first-rating protected bird in China, which has two subspecies, O. t. tarda and O. t. dybowskii. The genetic diversity and phylogenetic differences between the two Otis tarda subspecies were analyzed by the mtDNA control region method that on behalf of the maternal genetic character and the microsatellite method that on behalf of parental genetic character. The number of haplotype, Л, δ and К of O. t. tarda are higher than those of O. t. dybowskii among three sequences of mtDNA control region (CtrIaL/CtrIIoH、L438/H772 and LCR2a/HCR8), and significantly higher than the population in the northwest of Songliao Plain. The numbers of alleles, Ho and He of O. t. dybowskii are lower than those of O. t. tarda among three microsatellites (Otmic08, Otmic16 and Otmic26). Therefore, the genetic diversity of O. t. dybowskii is obviously lower than that of O. t. tarda. The two subspecies are located at two phylogenetic branches, which confirmed that the phylogenetic relationship between two subspecie.Moreover, O. t. tarda has much more phylogenetic branches than O.t. dybowskii.

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