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作 者 ：于倩,李明军

期 刊 ：生态学报 2004年 24卷 5期 页码：1022~1026

关键词：怀山药；微型块茎；愈伤组织；高频率再生；激素；暗培养；光培养；

Keywords:Dioscorea opposita Thunb, bulbils, callus, high-frequency regeneration, hormone, light culture, dark culture,

摘 要 ：对怀山药微型块茎愈伤组织的诱导及高频率再生进行了研究。结果表明 :(1)光下诱导铁棍山药脱分化形成愈伤组织 ,6 -BA2 mg/L+NAA2 mg/L 为最佳激素组合。KT2 mg/L+2 ,4 - D2 mg/L 有利于铁棍山药愈伤组织的增殖。附加 2 mg/L NAA能缩短 4 7号山药愈伤组织的诱导时间。KT2 mg/L+NAA2 m g/L 有利于 4 7号山药愈伤组织增殖 ;(2 )不同光照条件对愈伤组织的诱导和增殖影响不同。光照是缩短 4 7号山药愈伤组织诱导时间的另一因素。暗培养有利于愈伤组织的增殖 ,对 4 7号山药来说 ,暗培养下诱导率也较高 ;(3)基因型不同 ,愈伤组织类型不同 ,诱导率和不定芽分化率也有差异 ,4 7号山药高于铁棍山药 ;(4 )KT对 4 7号山药愈伤组织分化形成不定芽起主要作用 ,2 ,4 - D2 mg/L+KT2 mg/L 为最佳激素组合 ;(5 )光培养有利于不定芽的分化。

Abstract:Dioscorea opposita Thunb (Chinese Yam) is widely known for its medicinal value in the traditional Chinese medicine. However, continued vegetative propagation over the years has resulted in declines in both quality and yield of the plant, which calls for research effort into understanding the controlling factors on vegetative production of D. opposita. In order to determine the optimal conditions for dedifferentiation and differentiation of adventitious buds in D. opposita, we studied the effects of differe...

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