Abstract:RAPD markers were used to assess genetic variation within and among relict populations of the tree fern Alsophila spinulosa (Cyatheaceae) from Jian Feng Ling (JFL) and Ba Wang Ling (BWL) in Hainan Province,as well as Tang Lang Shan (TLS),Hei Shi Ding (HSD) and Da Xi Shan (DXS) in Guangdong Province of southern China.Twenty\|eight random primers detected 118 sites of which 33 (27 9%) were polymorphic.Calculations of Shannon diversity,Nei genetic differentiation,and AMOVA all consistently indicate that t...