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作 者 ：李光汉,杨智,黄祥明,刘选珍,余建秋,李学兵,方盛国,魏辅文,冯祚建

期 刊 ：生态学报 2000年 20卷 2期 页码：184~186

关键词：小熊猫；DNA指纹图；遗传多样性；影响因子；

Keywords:red panda（ Ailurus fulgens）, DNA fingerprinting, genetic diversity,

摘 要 ：利用寡核苷酸指纹探针 LZF- 检测了成都大熊猫繁育研究基地仿生态圈养场小熊猫种群全部 2 2只个体的 DNA指纹 ,检测结果表明其遗传多样性参数如下 :( 1 )各体间 DNA指纹图的相似性为 0 .1 72 ;个体 A的指纹谱带在个体 B中出现的概率为 0 .1 80 6。 ( 2 )圈养种群的等位基因频率为 0 .0 94 8,杂合率为 90 .52 %。 ( 3 )种群中指名亚种亲本个体的引进是影响该种群遗传多样性的主要因子。

Abstract:Using the method of labeling oligonucleotide probe LZF Ⅰ,we determined DNA fingerprints of 22 captive red pandas in our base to measure the genetic diversity of the captive population.The results were as follows similarity coefficient of DNA fingerprinting bands each individual was 0.172.Probability of fingerprinting bands of individual A which appeared in individual B was 0.1806.Mean allelic frequency of the captive population was 0.0948 and heterozygous ratio was 90.52%.Introducing subspecies fulgens to ...

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