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作 者 ：张弛， 潘家荣， 帅瑞琪， 方豪， 冯依璠

期 刊 ：核农学报 2016年 30卷 2期 页码：323-331

关键词：硝基咪唑类抗生素；酶联免疫分析；通用结构；

Keywords:nitroimidazoles, ELISA, common-structure antibody,

摘 要 ：本试验采用活化酯法,将合成的具有2-甲基-5-硝基咪唑通用结构的半抗原与载体蛋白偶联制备免疫抗原和包被抗原,以获得的抗原制备2-甲基-5-硝基咪唑通用结构抗体,并建立5种硝基咪唑类药物及代谢物的酶联免疫分析技术。结果表明:制备的抗体效价高于1∶100 000,该方法在不同动物源性食品中的加标回收率在85.65%~108.65%之间,检测限达1.9×10^-4mg·L^-1,对2-甲基-5-硝基咪唑、甲硝唑、替硝唑、地美硝唑和塞克哒唑 5种硝基咪唑抗生素及代谢物交叉反应率达95%以上。本研究为进一步建立和完善酶联免疫法检测动物性食品中硝基咪唑类药物残留提供了技术支撑。

Abstract:In this experiment, the 2-methyl-5-nitroimidazole hapten was synthesized, which was used to prepare the immune antigen and envelope antigen, with activated ester method to link to a carrier protein. And the hapten was identified. With the antigen prepared 2-methyl-5-nitroimidazole common antibody,the ELISA of 5 kinds of nitroimidazoles was established. The results showed that: An antiserum with titer of 1:100 000 was prepared and an ELISA for simultaneously detection of multi-residues of nitroimidazoles in foods of animal origin and recovery of 85.65%-108.459% had been established, the LOD was as low as 1.9×10^-4mg·L^-1. The cross-reaction rates to 2-methyl-5-nitroimidazole, metronidazole, tinidazole, dimetridazole and Secnidazole were above 95%. This study provided the technological base to the fast testing of veterinary drug residues in foods of animal origin.

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