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作 者 ：许嫚, 韩烨, 吕加平, 薛海晓, 逄晓阳, 张书文, 刘鹭

期 刊 ：核农学报 2016年 30卷 3期 页码：533-540

关键词：紫色杆菌素；脱氧紫色杆菌素；结构鉴定；理化性质；

Keywords:violacein, deoxyviolacein, structure identification, physicochemical properties,

摘 要 ：为探究产自细菌的蓝紫色天然色素的基本性质,采用高效液相色谱法,质谱、核磁共振分析等方法对嗜冷菌詹森杆菌产生的蓝紫色天然色素进行结构及理化性质分析。结果表明,该蓝紫色素由脱氧紫色杆菌素和紫色杆菌素2个色氨酸分子氧化缩合而成,比例约为1∶9。理化性质分析结果表明,该蓝紫色素热稳定性良好,光照及强碱对颜色有较大影响,易溶于甲醇、丙酮等有机溶剂,微溶于水;Cu²⁺对其稳定性影响较大。本研究为蓝紫色素在生物活性、抗肿瘤、抗病毒药物及生物染料等方面的研究和应用提供了基础。

Abstract:In order to explore the basic properties of the blue and purple nature pigments produced by bacteria.Experiments used high performance liquid chromatography (HPLC), mass spectrometry, and nuclear magnetic resonance (NMR) to analysis the physicochemical properties and chemical structure of the blue purple pigment producted by psychrophilic bacteria.The results showed that: the blue-purple pigment was verified to be composed of deoxidation violacein and violacein with the ratio of about 1∶9. The results of the physical and chemical properties of the blue purple pigment revealed that the purple pigment had good thermal stability, but light and strong alkali had a larger effect on color; soluble in methanol, acetone and other organic solvents, slightly soluble in water; copper ion has a great influence on its stability. This study provides a basis for the research and application of the biological activity, anti tumor, anti viral drugs and biological dyes,etc.

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