Abstract:In order to investigate effect of eco-planting and breeding of rice and frogs on the relation between soil nutrient supplying and the functions of microbiology the indexes, including the population of soil microorganisms, microbial activities, acid phosphatase activities, and inorganic phosphorus contents in treatment and control field, which was applied with pesticides and chemical fertilizers, were determined at different stages. The results indicated that the number of bacteria, actinomycetes and fungi increased with the prolonged periods after planting and breeding of rice and frogs. The microbial activities, the acid phosphatase activities, and the inorganic phosphorus contents in the treated group were significantly higher than these in the control group after the frogs were bred from 60 to 90 d. In addition, these dominant microorganisms benefited for the nutrition circulation, and the frog breeding promoted microbial activities, acid phosphatase activities, and soluble inorganic phosphorus contents of the soil. Thus, the eco-planting and breeding of rice and frog increased the number of soil microorganisms including Bacillus megatherium and microbial activities, improved acid phosphatase activities, promoted the transfer of indissolvable phosphorus to the dissolvable phosphorus, and finally increased the use of soil phosphorus. In conclusion, eco-planting and breeding of rice and frogs provided a new mode for increasing economic benefits of rice field.