Abstract:To screen functional genes related to resist rice sheath blight disease,, a gene encoding a nucleoporin (nup), named as OsSeh1, was cloned from rice Nipponbare (Oryza sativa L.spp.japonica,var nippobare) in this study. OsSeh1 shared identity with AtSeh1 of Arabidopsis thaliana, and Seh1 proteins from different plant species contained the same conservative domains. Protein OsSeh1 was observed exclusively in the nucleus of tobacco leaves. In rice plant, the expression of OsSeh1 gene was induced by salicylic acid or sheath blight agent Rhizoctonia solani. The expression of OsSeh1 at 48 hours point reached the peak under the induction of salicylic acid and the quantity was 2 times higher than that at 0 hour point. The highest expression quantity of OsSeh1 at 24 hours was as 3.5 times as that at 0 hour point, with treated with Rhizoctonia solani. 15 days after inoculation with Rhizoctonia solani, the disease degree results overall showed that T1 rice plants over-expression of OsSeh1 were more resistant to Rhizoctonia solani. On the contrast, RNA interference (RNAi) rice lines were more susceptible to Rhizoctonia solani. In T1 over-expression rice plants with significant resistance, relative expression quantity of OsSeh1 is 6 to 11, which higher than 2.47 in wild type rice Nipponbare. Otherwise, relative expression quantity of OsSeh1 is 0.2 to 0.6 in RNAi rice plants with obvious susceptibility. These results showed that rice resistance to Rhizoctonia solani was positively correlated with OsSeh1 expression levels. OsSeh1 was preliminarily proved to be an important gene in rice resistance to sheath blight,and this work will give a new thinking of improving sheath blight resistance by using rice genes resource.