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作 者 ：刘秀芳;林文革*;苏明华

期 刊 ：植物研究 2011年 31卷 5期 页码：569-574

关键词：樟；龙脑樟；组织培养；工厂化生产；

Keywords:Cinnamomum camphora, borneol camphor, tissue culture, industrialization production,

摘 要 ：选取龙脑含量高的优良单株，以其树干基部的幼嫩萌条为外植体开展龙脑樟组培快繁技术研究。结果表明：以改良MS+BA 2.0 mg·L^-1+NAA 0.1 mg·L^-1为芽诱导培养基，诱导率为93%；最佳增殖培养基为改良MS+BA 2.0 mg·L^-1 +NAA 0.05 mg·L^-1，增殖系数为5.57，生长周期为30 d；适宜的增殖培养条件为温度25℃，光照强度3 000 lx，光照时间11 h，不定芽长势良好；最佳生根培养基为1/2改良MS+IBA 0.5 mg·L^-1+IAA 0.4 mg·L^-1+蔗糖20 g·L^-1，生根率可达97.3%，生根条数为3～5条，生根时间为12 d；以草炭+珍珠岩(3∶1)为移栽基质，成活率可达86.2%。通过试验总结出一套组培快繁技术体系，可应用于龙脑樟组培苗工厂化生产。

Abstract:The techniques of tissue culture of Cinnamomum camphora(Linn.) Pves were developed from the explants taken from young sprouting branches at the base of plus tree with high contents of borneo camphor. The results showed that the adventitious buds were induced at the induction rate of 93% on modified MS supplemented with BA 2.0 mg·L^-1 and NAA 0.1 mg·L^-1. The modified MS adding BA 2.0 mg·L^-1 and NAA 0.05 mg·L^-1 was the optimized subculture medium with multiplication coefficient being 5.57 and the growth cycle being 30 d. The shoots grew well under the conditions of temperature 25℃, illumination intensity 3 000 lx and illumination time 11 h. They rooted in 12 d on 1/2 MS adding IBA 0.5 mg·L^-1, IAA 0.4 mg·L^-1 and sucrose 20 g·L^-1 with rooting rate reaching up to 97.33%, and the number of roots being 3 to 5. The plantlets were transplanted in mixture medium with peat and perlite(3∶1), and the survival rate was 86.2%. These techniques could be used in industrialized seedling production for the chemical form of this species.

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