Abstract:Cytological investigation on microsporogenesis of male sterility of pepper were studied, the results were as following: Abortion was found from multiplication stage of sporogenous cells.Sporogenous cells were vacuolated, misshapen, stretched-out and the clearance between cells was enlarged. Tapetum cells were elongated, vacuolated or tapetum was made up of multilayer cells or discomposed tardily. The structure of powder chamber was abnormal and replaced by indifferentiated cells full of thin cytoplasm. Cell layers of powder chamber wall and middlelevel were increased, tapetum cells were hypertrophied and sporogenous cells or microspore mother cells were disaggregated. Quartered microspore couldn’t be released because the callose wall of microspores couldn’t decompose. Cytoplasm of monocytic microspore were decompounded.