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作 者 ：孙红梅;袁思施;王春夏

期 刊 ：植物研究 2009年 29卷 1期 页码：54-57

关键词：百合；淀粉磷酸化酶；提取条件；测定条件；

Keywords:Lilium davidii var. unicolor, starch phosphorylase(SP), extractive condition, measurement condition,

摘 要 ：为深入研究百合鳞茎淀粉磷酸化酶（SP）的性质及其在鳞茎淀粉代谢中的生理作用，本试验确定了兰州百合鳞茎内SP的最佳提取和测定条件，并对部分酶学性质进行了初步探索。结果表明：SP的最适提取缓冲液为pH5.8的琥珀酸缓冲液；最佳反应体系为以25 mmol·L^-1的Glc-1-P为底物，在30℃条件下反应10 min。在pH5~6范围内，SP活性最高；百合鳞茎的SP不易保存且不耐热，4℃条件下保存12 h，酶活力最高残留50%；40℃条件下保存30 min后，SP活力几乎完全丧失。

Abstract:For a deep study on the characters of starch phosphorylase(SP) as well as its physiological effects during starch metabolism, the optimum extractive techniques and measurement conditions of SP in the bulb of Lilium davidii var. unicolor were ascertained, furthermore, some biochemical nature of this enzyme were preliminarily studied in this experiment. The results indicated that the best extractive buffer was succinic acid solution with pH 5.8. The optimal reaction system comprised 25 mmol·L^-1 Glc-1-P as substrate, and incubating for 10 min at 30℃. Within the pH scale from 5 to 6, SP presented higher activity. However, SP in lily bulb couldn’t be reserved for a long time and couldn’t sustain higher temperature. Stored for 12 hours at 4℃, SP activity only remained 50%, and almost lost completely after incubating for 30 min at 40℃.

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