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作 者 ：睢玉祥;张家铭;穆楠;杜贞;闫桂琴*

期 刊 ：植物研究 2010年 30卷 (3)期 页码：373-378

关键词：翅果油树；叶片；总黄酮；大孔吸附树脂；分离纯化；

Keywords:Elaeagnus mollis, leaves, total flavonoids, macroreticular absorption resin, separation and purification,

摘 要 ：

用紫外分光光度法测定翅果油树(Elaeagnus mollis Diels)叶总黄酮的含量，计算D4020、AB-8、S-8、X-5四种树脂的饱和吸附量和解吸率，比较分析各树脂对翅果油树叶总黄酮的分离纯化效果，确定最佳树脂及其最佳工艺条件。结果表明：S-8对翅果油树叶片总黄酮有高的吸附量（9.83 mg·g^-1），AB8次之（9.38 mg·g^-1），但解吸率AB-8（88.79%）高于S-8（71.89%）。确定所选树脂中AB-8为翅果油树叶总黄酮的最佳吸附剂，最佳工艺条件为：吸附液pH值3.0~4.0、上样量与树脂量之比6∶1、吸附流速2 mL·min^-1、洗脱液为60%乙醇、洗脱流速2 mL·min^-1、洗脱剂用量3BV。

Abstract:The content of total flavonoids in Elaeagnus mollis Diels leaves was determined by ultraviolet spectrophotometry,the adsorption capacity and desorption rate of four kinds of macroporous resins(D4020、AB-8、S-8、X-5) were calculated to determine the suitable resin and its optimum process conditions. The results indicated: S-8 resin had the highest adsorption capacity(9.83 mg·g^-1), followed by AB-8(9.38 mg·g^-1), but the desorption rate of AB-8 resin(88.79%) was better than that of S-8 resin(71.89%).AB-8 resin was the best absorbent for the total flavonoids in E.mollis leaves. The optimum process conditions were: the pH value of extracts was 3.0~4.0, the ratio of absorbing solution to resin was 6∶1, the adsorption flow rate was 2 mL·min^-1, the eluting solution was 60% ethanol, the eluting flow rate was 2 mL·min^-1, the volume of the eluting solution was 3BV.

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