---

作 者 ：徐凤萍;杨君*;程云清;刘建凤;安利佳

期 刊 ：植物研究 2009年 29卷 2期 页码：230-233

关键词：smGFP；直接转化；瞬时表达；线性化元件；

Keywords:smGFP, direct transformation, transient expression, minimal linear transformation cassette,

摘 要 ：通过转化缓冲溶液调整转化系统的渗透压，实现了含有smGFP基因的最小线性化元件在洋葱表皮细胞中的直接转化和瞬时表达。利用荧光显微镜在细胞质和细胞核中观察到很强的绿色荧光蛋白的表达，表明smGFP线性化元件顺利导入细胞内，并维持了表达元件的完整性。该方法仅通过缓冲溶液的设计实现外源基因的直接转化，无需基因枪、电激、注射等转化动力来源，能够显著减少实验操作对受体材料的破坏性，扩大转化受体的应用范围，同时操作简便快捷，大大降低了实验成本。

Abstract:The minimal linear smGFP gene cassette was constructed and transiently expressed in onion epidermal cell by adjusting the osmotic pressure of the transformation buffer. Strong fluorescence of green fluorescent protein was observed in cytoplasm and nucleus by fluorescence microscope, which proved that smGFP gene cassettes transformed into onion epidermal cell and kept its integrity. Exogenous gene was penetrated into host cell directly without any other force, such as particle bombardment, electransformation and microinjection. Therefore, it induces less damage to the host cells and has a wide range of application. In addition, this method is easily operated and less costly.

---